The virtues listed on Jacob's ladder are the three principal rounds: Faith, Hope, and Charity. These virtues are emphasized in Masonic teachings as essential qualities for personal development and spiritual ascent. The ladder symbolizes the journey of the soul towards perfection, with each rung representing a step in this journey.
As noted in the writings, "the three principal rounds of which are denominated Faith, Hope, and Charity; and which admonish us to have Faith in God, Hope in Immortality, and Charity to all mankind" .